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JoVE Journal Neuroscience
Fluorescence Activated Cell Sortering (FACS) og Gene Expression Analyse av Fos-uttrykker Nerveceller fra fersk og fryst Rat hjernevev
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Fluorescence Activated Cell Sortering (FACS) og Gene Expression Analyse av Fos-uttrykker Nerveceller fra fersk og fryst Rat hjernevev

Article DOI: 10.3791/54358-v • 08:37 min • August 27th, 2016
August 27th, 2016 •
*1, *1, 1, 2, 1
1Behavioral Neuroscience Research Branch, Intramural Research Program, NIDA, NIH, DHHS, 2Division of Rheumatology, School of Medicine, Johns Hopkins University
* These authors contributed equally

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Her presenterer vi en Fluorescence Activated Cell Sortering (FACS) protokoll for å studere molekylære endringer i Fos-uttrykke nevrale ensembler fra både fersk og frossen hjernevev. Bruken av frossent vev tillater FACS isolering av mange hjernen områder over flere økter for å maksimere bruken av verdifulle dyr fag.

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Neuroscience celletyper neuroner gliaceller antistoff mRNA FACS
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