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Journal / Genetics / Flow-Sortierung und Exome Sequenzierung der…
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JoVE Journal Genetics
Flow-Sortierung und Exome Sequenzierung der Reed-Sternberg-Zellen des klassischen Hodgkin-Lymphoms
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Flow-Sortierung und Exome Sequenzierung der Reed-Sternberg-Zellen des klassischen Hodgkin-Lymphoms

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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Hier beschreiben wir ein kombiniertes Durchflusszytometrisches Zellsortier- und Low-Input-Bibliothekskonstruktionsprotokoll der nächsten Generation, das für die Herstellung von hochwertigen, exakt exakten Daten aus den Hodgkin Reed-Sternberg (HRS) Zellen des klassischen Hodgkin-Lymphoms (CHL) entwickelt wurde.

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Genetik Ausgabe 124 Klassisches Hodgkin-Lymphom CHL Reed-Sternberg FACS Exome Sequenzbibliothek der nächsten Generation Durchflusszelle-Sortierung
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