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Journal / Genetics / 经典霍奇金淋巴瘤的芦苇细胞的流分选和外显子测序…
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JoVE Journal Genetics
经典霍奇金淋巴瘤的芦苇细胞的流分选和外显子测序
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经典霍奇金淋巴瘤的芦苇细胞的流分选和外显子测序

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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在这里，我们描述了一种组合流式细胞分选和低投入的下一代图书馆建设协议，旨在从古典霍奇金淋巴瘤（CHL）的霍奇金Reed-Sternberg（HRS）细胞产生高质量的全外显子数据。

Tags

遗传学，第124期，古典霍奇金淋巴瘤，cHL，Reed-Sternberg，FACS，外显子，下一代测序文库，流式细胞分选
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