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JoVE Journal Genetics
Classificação de fluxo e sequenciação Exome das células de Reed-Sternberg do linfoma de Hodgkin clássico
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Classificação de fluxo e sequenciação Exome das células de Reed-Sternberg do linfoma de Hodgkin clássico

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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Aqui, descrevemos uma classificação de células de citometria de fluxo combinado e um protocolo de construção de bibliotecas de próxima geração, de baixa entrada, projetado para produzir dados de alta qualidade e total de exomas das células Hodgkin Reed-Sternberg (HRS) do linfoma de Hodgkin clássico (CHL).

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Genetics linfoma de Hodgkin clássico cHL Reed-Sternberg FACS exome biblioteca de sequenciação de próxima geração classificação de células de fluxo
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