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CUBIC protokol Visualiserer Protein Expression på Single Cell Opløsning i Whole Mount Skin Forberedelser
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CUBIC protokol Visualiserer Protein Expression på Single Cell Opløsning i Whole Mount Skin Forberedelser

Article DOI: 10.3791/54401-v • 08:09 min • August 4th, 2016
August 4th, 2016 •
*1,2, *1, *1, 3, 1, 1
1The School of Medical Sciences, University of New South Wales Australia, 2Neuroscience Research Australia, 3Biomedical Imaging Facility, University of New South Wales Australia
* These authors contributed equally

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Denne rapport beskriver en CUBIC protokol at afklare fuld tykkelse mus hud biopsier, og visualisere protein ekspressionsmønstre, prolifererende celler og sebocytter på enkelt celle opløsning i 3D. Denne metode giver nøjagtig vurdering af hudens anatomi og patologi, og af unormale epidermale fænotyper i genetisk modificerede mus linjer.

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Cellular Biology hud epidermis CUBIC 3D billedbehandling konfokal mus
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