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使用F9稀土LACZ基于Cell的报告基因检测在E8.5胚胎和神经球文化相对维甲酸水平的定量测量
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay
Please note that all translations are automatically generated. Click here for the English version.

使用F9稀土LACZ基于Cell的报告基因检测在E8.5胚胎和神经球文化相对维甲酸水平的定量测量

DOI:
10.3791/54443-v

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09:49 min

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September 06, 2016

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, , ,
1Center for Advanced Biotechnology and Medicine,Rutgers University, 2Department of Neuroscience and Cell Biology,Rutgers University

Chapters

  • 00:05Title
  • 01:08Subcloning of F9 RARE-LacZ Cells
  • 03:03F9 RARE-LacZ Plate Preparation
  • 04:08Co-culture and Standard Curve Set Up
  • 06:08RA Assay
  • 07:31Results: Analyzing RA-levels from Gpr161vl/vl E8.5 and Neurosphere Cells
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

Methods to accurately measure retinoic acid (RA) levels in small amounts of tissue do not exist. This protocol describes the easy, quantitative measurement of relative RA levels in E8.5 embryos and neurospheres using an RA reporter cell line.

Tags

Quantitative MeasurementRelative Retinoic Acid LevelsE8.5 EmbryosNeurosphere CulturesF9 RARE-LacZ Cell-based Reporter AssayRA Signaling PathwayRA Metabolic EnzymesRA ConcentrationLacZ StainingGelatin-coated PlatesCell CultureColony SelectionCell Expansion

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