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Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
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Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

Article DOI: 10.3791/54460-v • 07:38 min • September 13th, 2016
September 13th, 2016 •
1,2, 1, 3, 4, *3, *1
1Department of Medicine B, University Hospital Münster, 2Institute of Palliative Care, University Hospital Münster, 3Biomedical Technology Center, University of Münster, 4Department of Gastroenterology, Klinikum Bielefeld
* These authors contributed equally

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Summary
Automatic Translation

Accurate assessment of anti-inflammatory effects is of utmost importance for the evaluation of potential new drugs for the treatment of inflammatory bowel disease. Digital holographic microscopy provides assessment of inflammation in murine and human colonic tissue samples as well as automated multimodal evaluation of epithelial wound healing in vitro.

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Multimodal Quantitative Phase Imaging Digital Holographic Microscopy Intestinal Inflammation Epithelial Wound Healing Automated Stain-free Quantitative Phase Contrast Imaging Gastrointestinal Inflammation Disease Activity Cell And Tissue Detection Physical Parameters Biomedical Technology Center Cell Culture Caco Two Cells Petri Dishes RPMI Medium FBS Antibiotics Agent Of Interest Incubator Supernatant Inserts HEPES Buffered Cell-cultured Medium
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