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Biochemistry

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JoVE Journal Biochemistry
Einzelmolekül-Super-Resolution Imaging von Phosphatidylinositol 4,5-bisphosphat in der Plasmamembran mit neuartigen Fluoreszenzsonden
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Einzelmolekül-Super-Resolution Imaging von Phosphatidylinositol 4,5-bisphosphat in der Plasmamembran mit neuartigen Fluoreszenzsonden

Article DOI: 10.3791/54466-v • 07:26 min • October 15th, 2016
October 15th, 2016 •
1, 1
1Department of Neuroscience, University of Wisconsin-Madison

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PI (4,5) P 2 regelt verschiedene Zellfunktionen, aber die nanoskaligen Organisation in der Zellplasmamembran ist wenig bekannt. Durch Markierung PI (4,5) P 2 mit einer zweifarbige Fluoreszenzsonde zur Domäne Pleckstrin Homology fusioniert beschreiben wir einen neuen Ansatz der PI (4,5) P 2 in der räumlichen Verteilung der Plasmamembran im Nanometerbereich zu studieren .

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Biochemie Heft 116 Super-Resolution-Bildgebung PALM Phosphatidylinositol 4,5-bisphosphat (PI (4,5) P2) Zellmembran Blatt Phosphoinositid Beta-Zellen
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