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JoVE Journal Developmental Biology
Manipolazione Ploidia di embrioni di zebrafish con Heat Shock 2 Trattamento
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Manipolazione Ploidia di embrioni di zebrafish con Heat Shock 2 Trattamento

Article DOI: 10.3791/54492-v • 11:19 min • December 16th, 2016
December 16th, 2016 •
1, *1,2, *1,3, 1
1Laboratory of Genetics, University of Wisconsin, 2Department of Neurobiology, University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program, University of Pittsburgh School of Medicine
* These authors contributed equally

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Un protocollo modificato per la manipolazione ploidia utilizza uno shock termico per indurre una stalla di un ciclo in cytokinesis in embrione precoce. Questo protocollo è illustrato nel zebrafish, ma può essere applicabile ad altre specie.

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Biologia dello Sviluppo Numero 118 zebrafish manipolazione ploidia intero genoma di duplicazione gynogenesis shock termico la duplicazione centriolo diploide omozigote
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