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JoVE Journal
Biology

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成像G蛋白偶联受体介导的趋化和信令活动在中性粒细胞样细胞HL60
 
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成像G蛋白偶联受体介导的趋化和信令活动在中性粒细胞样细胞HL60

Article DOI: 10.3791/54511-v 08:24 min September 14th, 2016
September 14th, 2016

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Summary

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Click here for the English version.

视觉趋化实验是为了更好地了解如何真核细胞趋化控制介导的定向细胞迁移是必不可少的。在这里,我们描述了详细的方法:1)实时,多趋化实验的高分辨率监测,和2)同时可视化趋化梯度和信号中的中性粒细胞样HL60细胞事件的时空动力学。

Tags

细胞生物学,第115,中性粒细胞趋化性,G蛋白偶联受体(GPCR),G-蛋白,信号转导,细胞迁移
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