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Indução da Aguda Skeletal regeneração muscular por cardiotoxina Injection
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Indução da Aguda Skeletal regeneração muscular por cardiotoxina Injection

Article DOI: 10.3791/54515-v • 07:39 min • January 1st, 2017
January 1st, 2017 •
1, 1, 2,3, 1, 2, 1
1Stem Cell Fate Laboratory, Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR, 2School of Medicine and Surgery, University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute

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Este manuscrito descreve um protocolo detalhado para induzir a regeneração do músculo esquelético aguda em ratos adultos e manipulações subsequentes dos músculos, como dissecção, corte, congelação, coloração de rotina e análise de células musculares área de secção transversal.

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Biologia do Desenvolvimento Edição 119 cardiotoxina Aguda Skeletal regeneração muscular tibial anterior Isolamento muscular Técnica Muscle Congelado Fresco Hematoxilina & eosina miofibras Área seccional transversa.
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