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Journal / Biochemistry / Proteinkomplex Affinity Capture från Cryomilled…
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Biochemistry

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JoVE Journal Biochemistry
Proteinkomplex Affinity Capture från Cryomilled däggdjursceller
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Proteinkomplex Affinity Capture från Cryomilled däggdjursceller

Article DOI: 10.3791/54518-v • 10:37 min • December 9th, 2016
December 9th, 2016 •
1,2, 1, 1
1Laboratory of Cellular and Structural Biology, The Rockefeller University, 2Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine

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Här beskriver vi protokoll för att störa däggdjursceller genom solid-state fräsning vid en kryogen temperatur, producerar ett cellextrakt från den resulterande cellpulver, och isolera proteinkomplex av intresse genom affinitetsinfångning vid antikroppsförenade mikron-skaleparamagnetiska pärlor.

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Biokemi affinitetsinfångning affinitetsrening immunfällning IP cryomilling störningar cell proteinkomplex rening
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