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Journal / Biochemistry / Yöntem HCN1 ve TRIP8b arasında protein-protein…
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Biochemistry

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JoVE Journal Biochemistry
Yöntem HCN1 ve TRIP8b arasında protein-protein etkileşimi küçük molekül önleyicilerinin tespit edilmesi için
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Yöntem HCN1 ve TRIP8b arasında protein-protein etkileşimi küçük molekül önleyicilerinin tespit edilmesi için

Article DOI: 10.3791/54540-v • 10:20 min • November 11th, 2016
November 11th, 2016 •
*1, *1, 2, 3, 1, 1, 4, 1,5
1Davee Department of Neurology and Clinical Neurosciences, Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery, Northwestern University, 3Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences, Northwestern University, 5Department of Physiology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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HCN kanalları ve bunların yardımcı alt birimi arasındaki etkileşim Majör Depresif Bozukluk bir tedavi hedefi olarak tespit edilmiştir. Burada, bu protein-protein etkileşimi, küçük molekül inhibitörleri belirlemek için bir floresan polarizasyon tabanlı bir yöntem sunulmuştur.

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Biochemistry Sayı 117 yüksek verimli tarama ilaç keşfi protein-protein etkileşimleri floresan polarizasyon HCN TRIP8b
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