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Journal / Immunology and Infection / Eksperimentell infeksjon med
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Immunology and Infection

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JoVE Journal Immunology and Infection
Eksperimentell infeksjon med
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Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
Journal (Immunology and Infection)
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
 
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Eksperimentell infeksjon med

Article DOI: 10.3791/54554-v • 10:10 min • November 16th, 2016
November 16th, 2016 •
*1, *1, *1, 1, 1,2,3
1Department of Immunology, University of Toronto, 2Toronto General Research Institute, University Health Network, 3Women's College Research Institute
* These authors contributed equally

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Summary
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Denne protokollen beskriver hvordan å inokulere C57BL / 6J-mus med EGD stamme av Listeria monocytogenes (L. monocytogenes) og for å måle interferon-γ (IFN-γ) responser av naturlige dreperceller (NK) celler, naturlig drepe-T (NKT-celler), og adaptive T-lymfocytter post-infeksjon. Denne protokollen beskriver også hvordan å gjennomføre overlevelse hos mus etter infeksjon med en modifisert LD 50 dose av organismen.

Tags

Infeksjon , Bakterier infeksjonsmodell mus strømningscytometri interferon-γ svar
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