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Métodos para o isolamento, Cultura e caracterização funcional de SINOATRIAL Nó miócitos de ratos adultos
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Métodos para o isolamento, Cultura e caracterização funcional de SINOATRIAL Nó miócitos de ratos adultos

Article DOI: 10.3791/54555-v • 09:32 min • October 23rd, 2016
October 23rd, 2016 •
*1, *1,2, 1,3
1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, 2Department of Bioengineering, University of Colorado Anschutz Medical Campus, 3Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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Métodos são demonstradas para o isolamento de miócitos do nó sinoatrial (SAM) a partir de ratinhos adultos por patch-clamp electrofisiologia ou imagiologia estudos. As células isoladas podem ser utilizadas directamente ou podem ser mantidas em cultura que permita a expressão de proteínas de interesse, tais como repórteres geneticamente codificados.

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Biologia Celular Edição 116 nó sinusal pacemaking cultura de células adenovírus eletrofisiologia atual engraçado
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