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Journal / Immunology and Infection / Fenotypisk Karakterisering av makrofager fra Rat…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Fenotypisk Karakterisering av makrofager fra Rat Nyre ved flowcytometri
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Fenotypisk Karakterisering av makrofager fra Rat Nyre ved flowcytometri

Article DOI: 10.3791/54599-v • 07:14 min • October 18th, 2016
October 18th, 2016 •
1, 1, 2, 3, 2, 2, 3, 3, 3, 1, 1
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

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Dette manuskriptet beskriver en detaljert protokoll for fenotypisk og kvantitativ analyse av fastboende makrofager fra rotte-nyrer ved strømningscytometri. De resulterende fargede celler kan også brukes til andre formål, inkludert celle sortering, genekspresjonsanalyser eller funksjonelle studier, og dermed øke den informasjon som er innhentet i den eksperimentelle modellen.

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Immunologi makrofager Betennelse M1 M2 fenotyper Polarisering Nyre Rotte
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