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Journal / Immunology and Infection / Metoder til at studere Lipidforandringer i…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Metoder til at studere Lipidforandringer i neutrofiler og den efterfølgende dannelse af neutrofile ekstracellulær fælder
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Metoder til at studere Lipidforandringer i neutrofiler og den efterfølgende dannelse af neutrofile ekstracellulær fælder

Article DOI: 10.3791/54667-v • 10:58 min • March 29th, 2017
March 29th, 2017 •
*1,2, *1,3, 1, 1,4, 1, 1,4
1Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 2Fish Disease Research Unit, University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center, Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover
* These authors contributed equally

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Lipider er kendt for at spille en vigtig rolle i cellulære funktioner. Her beskriver vi en fremgangsmåde til bestemmelse lipidsammensætningen af ​​neutrofiler, med vægt på kolesterolniveauet, ved hjælp af både HPTLC og HPLC at få en bedre forståelse af de underliggende mekanismer for neutrofil ekstracellulær fælde dannelse.

Tags

Immunologi Neutrofiler Neutrofil Ekstracellulær Fælder (NET) kolesterol methyl-β-cyclodextrin (MβCD) High Performance Liquid Chromatography (HPLC) High Performance Tyndtlagskromatografi (HPTLC)
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