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Journal / Immunology and Infection / Methoden om te studeren Lipidenveranderingen in…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Methoden om te studeren Lipidenveranderingen in neutrofielen en de daaropvolgende vorming van neutrofielen Extracellulaire Traps
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Journal (Immunology and Infection)
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Methoden om te studeren Lipidenveranderingen in neutrofielen en de daaropvolgende vorming van neutrofielen Extracellulaire Traps

Article DOI: 10.3791/54667-v • 10:58 min • March 29th, 2017
March 29th, 2017 •
*1,2, *1,3, 1, 1,4, 1, 1,4
1Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 2Fish Disease Research Unit, University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center, Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover
* These authors contributed equally

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Lipiden is bekend dat ze een belangrijke rol in cellulaire functies spelen. We beschrijven een werkwijze om de lipidesamenstelling van neutrofielen te bepalen, met de nadruk op het cholesterolgehalte, door zowel HPTLC en HPLC aan een beter begrip van de onderliggende mechanismen van neutrofielen extracellulaire val vorming krijgen.

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Immunologie neutrofielen neutrofiel Extracellulaire Traps (NET) cholesterol Methyl-β-cyclodextrine (MβCD) hogedrukvloeistofchromatografie (HPLC) High Performance dunnelaagchromatografie (HPTLC)
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