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Journal / Immunology and Infection / Metoder for å studere Lipidendringer av…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Metoder for å studere Lipidendringer av nøytrofile granulocytter og etterfølgende dannelse av Neutrofil Ekstracellulær feller
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Metoder for å studere Lipidendringer av nøytrofile granulocytter og etterfølgende dannelse av Neutrofil Ekstracellulær feller

Article DOI: 10.3791/54667-v • 10:58 min • March 29th, 2017
March 29th, 2017 •
*1,2, *1,3, 1, 1,4, 1, 1,4
1Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 2Fish Disease Research Unit, University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center, Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover
* These authors contributed equally

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Lipider er kjent for å spille en viktig rolle i cellulære funksjoner. Her beskriver vi en fremgangsmåte for å bestemme lipid sammensetningen av neutrofiler, med vekt på kolesterolnivået, ved å bruke både HPTLC og HPLC for å få en bedre forståelse av de underliggende mekanismene for nøytrofil ekstracellulær felle dannelse.

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Immunology utgave 121 neutrofiler Neutrofil Ekstracellulær feller (garn) kolesterol metyl-β-cyklodekstrin (MβCD) High Performance Liquid Chromatography (HPLC) høy-ytelses tynnsjiktkromatografi (HPTLC)
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