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Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons
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JoVE Journal Neuroscience
Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

DOI:
10.3791/54685-v

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09:04 min

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September 14, 2016

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, , , ,
1IFIBYNE-CONICET,University of Buenos Aires, 2Center for Translational Neuroscience,University of Arkansas for Medical Sciences

Chapters

  • 00:05Title
  • 00:54Solutions Preparation
  • 02:07Slice Preparation
  • 03:12Whole-Cell Patch Clamp Recordings
  • 06:32Results: Gamma Band Membrane Oscillations in Whole-Cell Recorded PPN Neurons Using Current Ramps of Augmenting Amplitude
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

The pedunculopontine nucleus (PPN) is located in the brainstem and its neurons are maximally activated during waking and rapid eye movement (REM) sleep brain states. This work describes the experimental approach to record in vitro gamma band subthreshold membrane oscillation in PPN neurons.

Tags

Keywords: Gamma Band OscillationsPedunculopontine Nucleus NeuronsIntrinsic Membrane PropertiesWhole-cell Patch Clamp RecordingACSF PreparationSucrose ACSFVibratome SlicingBrain Dissection

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