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Journal / Immunology and Infection / Generering
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Immunology and Infection

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Generering

Article DOI: 10.3791/54697-v • 08:48 min • October 25th, 2016
October 25th, 2016 •
1,2, *2, *2, 2, 1,2, 1,2, 2, 2, 1,2, 1,2
1Department of Immunology, University of Toronto, 2Princess Margaret Cancer Centre, University Health Network
* These authors contributed equally

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Heri beskriver vi en hidtil ukendt fremgangsmåde til at generere antigenspecifikke T-celle-receptorer (TCR'er) ved parring af TCRα eller TCRβ af en eksisterende TCR, der besidder den antigen-specificitet af interesse, med komplementær hemichain af den perifere T-celle receptor repertoire. De novo genererede TCR'er bevarer antigen-specificitet med varierende affinitet.

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Immunologi T-celle antigenpræsenterende celle T-celle-receptor hemichain retroviral transduktion antigen-specificitet human kloning
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