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Journal / Immunology and Infection / Generering
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Immunology and Infection

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Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Journal (Immunology and Infection)
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Journal (Immunology and Infection)
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
 
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Generering

Article DOI: 10.3791/54697-v • 08:48 min • October 25th, 2016
October 25th, 2016 •
1,2, *2, *2, 2, 1,2, 1,2, 2, 2, 1,2, 1,2
1Department of Immunology, University of Toronto, 2Princess Margaret Cancer Centre, University Health Network
* These authors contributed equally

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Summary
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Heri vi beskrive en ny fremgangsmåte for å generere antigen-spesifikke T-celle-reseptorer (TCR) etter sammenkobling TCRα eller TCRβ av en eksisterende TCR, som har antigen-spesifisitet av interesse, med komplementære hemichain av den perifere T-cellereseptoren repertoar. De novo generert TCR beholde antigen-spesifisitet med varierende affinitet.

Tags

Immunology T-celle antigen-presenterende celle T-celle-reseptor hemichain retroviral transduksjon antigen-spesifisitet human kloning
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