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Journal / Biology / Analisi di SCAP
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JoVE Journal Biology
Analisi di SCAP
Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation
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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Journal (Biology)
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Analisi di SCAP

Article DOI: 10.3791/54709-v • 11:27 min • November 8th, 2016
November 8th, 2016 •
1, 1, 1, 1, 1, 1, 1
1Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center and College of Medicine

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Descriviamo un metodo modificato per l'isolamento frazione di membrana da cellule umane e preparazione del campione per la rilevazione di -glycosylation SCAP N e proteine totali mediante western blot. Introduciamo inoltre un metodo GFP-etichettatura per monitorare il traffico di SCAP usando la microscopia confocale. Questo protocollo può essere utilizzato in laboratori di biologia regolare.

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Biologia Cellulare Numero 117 SCAP, SREBPs reticolo endoplasmatico proteina di membrana la sintesi dei lipidi
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