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SCAP分析
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SCAP分析

Article DOI: 10.3791/54709-v • 11:27 min • November 8th, 2016
November 8th, 2016 •
1, 1, 1, 1, 1, 1, 1
1Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center and College of Medicine

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我们通过使用蛋白质印迹描述了从人类细胞的膜级分的分离和样品制备用于检测SCAPÑ-glycosylation和总蛋白的改进方法。我们还引进了GFP标记法使用共聚焦显微镜监测SCAP贩卖。这种协议可以在常规生物学实验室使用。

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细胞生物学，第117，SCAP，
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