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Immunology and Infection

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JoVE Journal Immunology and Infection
En enkel Fluorescens analyse for Kvantifisering av Canine nøytrofile ekstracellulær felle utløsning
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En enkel Fluorescens analyse for Kvantifisering av Canine nøytrofile ekstracellulær felle utløsning

Article DOI: 10.3791/54726-v • 06:45 min • November 21st, 2016
November 21st, 2016 •
1, 2, 3
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, 2MRC Centre for Inflammation Research, University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University

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Nøytrofile ekstracellulære feller (nett) er nettverk av DNA, histoner og nøytrofile proteiner. Selv om en komponent av den medfødte immunrespons, er garn implisert i autoimmunitet og trombose. Denne protokollen beskriver en enkel metode for canine nøytrofil isolering og kvantifisering av nett ved hjelp av en mikroplate-fluorescens-analyse.

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Immunologi hund NET nøytrofile ekstracellulære felle fluorescens mikroanalyse hjørnetann
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