Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Tre-dimensionel Super Resolution Mikroskopi af…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Tre-dimensionel Super Resolution Mikroskopi af F-actinfilamenter af Interferometrisk fotoaktiverede Lokalisering Microscopy (iPALM)
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Journal (Biology)
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Rat Mesentery Angiogenesis Assay
Journal (Biology)
Rat Mesentery Angiogenesis Assay
 
Click here for the English version

Tre-dimensionel Super Resolution Mikroskopi af F-actinfilamenter af Interferometrisk fotoaktiverede Lokalisering Microscopy (iPALM)

Article DOI: 10.3791/54774-v • 11:57 min • December 1st, 2016
December 1st, 2016 •
1, 2,3
1Department of Biology, South University of Science and Technology of China, Shenzhen, 2Mechanobiology Institute, Singapore, 3Department of Biomedical Engineering, National University of Singapore

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi præsenterer en protokol for anvendelse af interferometrisk fotoaktiverede Lokalisering Microscopy (iPALM), en 3-dimensionel single-molekyle lokalisering super opløsning mikroskopi metode til billeddannelse af actincytoskelettet i vedhængende pattedyrceller. Denne fremgangsmåde giver lys-baserede visualisering af nanostørrelse strukturelle træk, der ellers ville forblive uløste ved konventionel diffraktionsbegrænset optisk mikroskopi.

Tags

Biofysik Super opløsning mikroskopi PALM iPALM Fluorescens F-actin Single Molecule Lokalisering Photoswitchable Fluoroforer interferometri
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter