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Tredimensjonal Super Resolution Mikroskopi av F-aktin filamenter av Interferometric PhotoActivated Lokalisering Mikroskopi (iPALM)
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Tredimensjonal Super Resolution Mikroskopi av F-aktin filamenter av Interferometric PhotoActivated Lokalisering Mikroskopi (iPALM)

Article DOI: 10.3791/54774-v • 11:57 min • December 1st, 2016
December 1st, 2016 •
1, 2,3
1Department of Biology, South University of Science and Technology of China, Shenzhen, 2Mechanobiology Institute, Singapore, 3Department of Biomedical Engineering, National University of Singapore

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Vi presenterer en protokoll for anvendelse av interferometriske PhotoActivated Localization Mikroskopi (iPALM), en tre-dimensjonal enkelt-molekyl lokalisering super oppløsning mikroskopi fremgangsmåte til avbildning av aktin cytoskjelettet i adherente pattedyrceller. Denne fremgangsmåten gjør det mulig for lys-basert visualisering av nanoskala strukturelle trekk som ellers ville forbli uløst ved konvensjonell diffraksjon begrenset optisk mikroskopi.

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Biofysikk Super oppløsning mikroskopi PALM iPALM fluorescens F-aktin enkelt molekyl lokalisering Photoswitchable fluorophores Interferometri
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