Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Structural Information from Single-molecule FRET…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Journal (Biochemistry)
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
In Vitro Polymerization of F-actin on Early Endosomes
Journal (Biochemistry)
In Vitro Polymerization of F-actin on Early Endosomes
 

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Article DOI: 10.3791/54782-v • 12:30 min • February 9th, 2017
February 9th, 2017 •
*1, *1, 1, 1, 1
1Institute of Biophysics, Ulm University
* These authors contributed equally

Chapters

Summary
Automatic Translation

We present the setup and experimental procedure to obtain smFRET data from large donor-acceptor networks with a TIRF microscope. The step-by-step analysis of these measurements with the Bayesian inference software Fast-NPS yields high-resolved structural information via the application of adapted dye models.

Tags

Fast Nano-positioning System Single-molecule FRET Experiments Structural Information Biomolecules Statistical Analysis Flexible Domains Macromolecules Dye Models Three-dimensional Probability Distribution Protein Database Quantitative Single-molecule Fluorescence Measurements Flow Chamber Sample Holder Inlet And Outlet Screws Silicone Tubing Quartz Slide Holes Glass Holder Screws Wash Neutravidin Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter