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Journal / Biochemistry / Thermostabilization, Expression, rening och…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Thermostabilization, Expression, rening och kristallisation av den mänskliga Serotonin Transporter Bound till
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Journal (Biochemistry)
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Manually Extracting the Bradford Assay Data Recorded with a Smartphone
Journal (Biochemistry)
Manually Extracting the Bradford Assay Data Recorded with a Smartphone
 
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Thermostabilization, Expression, rening och kristallisation av den mänskliga Serotonin Transporter Bound till

Article DOI: 10.3791/54792-v • 12:21 min • November 27th, 2016
November 27th, 2016 •
1, 2, 1,3
1Vollum Institute, Oregon Health & Science University, 2Graduate Group in Biophysics, University of California, San Francisco, 3Howard Hughes Medical Institute, Oregon Health & Science University

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Detta manuskript beskriver hur man screena för thermostabilizing mutationer, rena mänskliga serotonintransportören, generera antikroppar med hög affinitet, och kristallisera serotonintransportören-antikroppskomplex bundet till det antidepressiva läkemedlet S -citalopram. Detta protokoll kan anpassas till studier av andra utmanande membrantransportörer, receptorer, och kanaler.

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Biokemi strukturbiologi kristallisering membranprotein antikropp immunisering beredning serotonintransportören signalsubstans antidepressiva selektiva serotoninåterupptagshämmare termo
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