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Journal / Biochemistry / Termostabilisering, Expression, Rensning, og…
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Biochemistry

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JoVE Journal Biochemistry
Termostabilisering, Expression, Rensning, og Krystallisation af Human Serotonin Transporter Bundet til
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
Journal (Biochemistry)
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
Journal (Biochemistry)
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Termostabilisering, Expression, Rensning, og Krystallisation af Human Serotonin Transporter Bundet til

Article DOI: 10.3791/54792-v • 12:21 min • November 27th, 2016
November 27th, 2016 •
1, 2, 1,3
1Vollum Institute, Oregon Health & Science University, 2Graduate Group in Biophysics, University of California, San Francisco, 3Howard Hughes Medical Institute, Oregon Health & Science University

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Dette håndskrift beskriver, hvordan man screene for thermostabilizing mutationer, oprense det humane serotonin transporteren, generere antistoffer med høj affinitet, og krystallisere serotonintransporteren-antistof komplekset bundet til det antidepressive lægemiddel S -citalopram. Denne protokol kan tilpasses til studiet af andre udfordrende membrantransportører, receptorer og kanaler.

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Biokemi strukturel biologi krystallisering membranprotein antistof immunisering rekonstituering serotonin transporteren neurotransmitter antidepressive selektive serotoningenoptagshæmmere termostabilitet
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