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Immunology and Infection

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JoVE Journal Immunology and Infection
SILAC Based Proteomic Charakterisierung von Exosomen aus HIV-1-infizierten Zellen
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SILAC Based Proteomic Charakterisierung von Exosomen aus HIV-1-infizierten Zellen

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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Hier beschreiben wir eine quantitative Proteomik Verfahren die Technik der Silac (SILAC) mit Hilfe der Wirkung von HIV-1-Infektion auf Host exosomalen Proteome zu analysieren. Dieses Protokoll kann leicht an Zellen unter verschiedenen Stress oder Infektionsbedingungen angepasst werden.

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Die Infektion Ausgabe 121 Exosomen extrazelluläre Fahrzeuge HIV-1 Proteomics Massenspektrometrie SILAC Bioinformatik
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