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Immunology and Infection

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JoVE Journal Immunology and Infection
SILAC Based proteomica Caratterizzazione di Esosomi da HIV-1 le cellule infette
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SILAC Based proteomica Caratterizzazione di Esosomi da HIV-1 le cellule infette

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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Qui, descriviamo un metodo di proteomica quantitativa utilizzando la tecnica di etichettatura degli isotopi stabili da aminoacidi nelle cellule in coltura (SILAC) per analizzare gli effetti di HIV-1 su proteomi exosomal host. Questo protocollo può essere facilmente adattato alle cellule in diverse condizioni di stress o infezione.

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L'infezione esosomi extracellulari Veicoli HIV-1 la proteomica la spettrometria di massa SILAC Bioinformatica
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