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Journal / Immunology and Infection / SILAC basert proteomikk Karakterisering av…
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Immunology and Infection

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JoVE Journal Immunology and Infection
SILAC basert proteomikk Karakterisering av Exosomes fra HIV-1 infiserte celler
Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)
Journal (Immunology and Infection)
Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
Journal (Immunology and Infection)
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
 
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SILAC basert proteomikk Karakterisering av Exosomes fra HIV-1 infiserte celler

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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Her beskriver vi en kvantitativ proteomikk metode ved hjelp av teknikken av stabile isotoper merking av aminosyrer i cellekultur (SILAC) for å analysere virkningene av HIV-1-infeksjon på verten exosomal proteom. Denne protokollen kan enkelt tilpasses til cellene under ulike stress eller smitteforhold.

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Infeksjon Exosomes ekstracellulære Kjøretøy HIV-1 proteomikk massespektrometri SILAC bioinformatikk
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