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Immunology and Infection

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JoVE Journal Immunology and Infection
SILAC Based proteômica Caracterização de exossomas de HIV-1 células infectadas
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SILAC Based proteômica Caracterização de exossomas de HIV-1 células infectadas

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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Aqui, nós descrevemos um método proteómica quantitativa utilizando a técnica de marcação por isótopos estáveis ​​aminoácidos em cultura de células (SILAC) para analisar os efeitos da infecção por HIV-1 em proteomas exosomal hospedeiro. Este protocolo pode ser facilmente adaptado a diferentes células sob condições de stress ou infecções.

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Infecção Edição 121 exossomos extracelulares Veículos HIV-1 proteômica espectrometria de massa SILAC Bioinformatics
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