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Immunology and Infection

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JoVE Journal Immunology and Infection
SILAC Based proteomiske Karakterisering af Exosomer fra HIV-1 inficerede celler
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SILAC Based proteomiske Karakterisering af Exosomer fra HIV-1 inficerede celler

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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Her beskriver vi en kvantitativ proteomics metode ved hjælp af teknikken af ​​stabile isotoper mærkning af aminosyrer i cellekultur (SILAC) at analysere virkningerne af HIV-1 infektion på host exosomal proteomer. Denne protokol kan let tilpasses til celler under forskellige stress eller infektion betingelser.

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Infektion exosomer Ekstracellulære Køretøjer HIV-1 Proteomics massespektrometri SILAC Bioinformatik
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