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Journal / Immunology and Infection / SILAC Based Proteoom karakterisatie van exosomen…
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Immunology and Infection

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JoVE Journal Immunology and Infection
SILAC Based Proteoom karakterisatie van exosomen van HIV-1 geïnfecteerde cellen
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SILAC Based Proteoom karakterisatie van exosomen van HIV-1 geïnfecteerde cellen

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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We beschrijven een kwantitatieve proteomics met gebruik van technieken van stabiele isotopen van aminozuren in celcultuur (SILAC) het effect van HIV-1 infectie op host exosomaal proteoom analyse. Dit protocol kan gemakkelijk worden aangepast aan cellen onder verschillende stress of infectie omstandigheden.

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Infectie exosomes extracellulaire Voertuigen HIV-1 Proteomics massaspectrometrie SILAC Bioinformatics
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