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Journal / Developmental Biology / Analyse af koronarkar i Cleared Embryonale hjerter
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Developmental Biology

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JoVE Journal Developmental Biology
Analyse af koronarkar i Cleared Embryonale hjerter
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Analyse af koronarkar i Cleared Embryonale hjerter

Article DOI: 10.3791/54800-v • 08:25 min • December 7th, 2016
December 7th, 2016 •
1, 1, 2, 1
1Developmental Biology of Birth Defects, UCL Institute of Child Health, 2MRC Centre for Regenerative Medicine, SCRM Building, University of Edinburgh

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Vi præsenterer en protokol til analyse af koronarkar i hele embryonale murine hjerter op til E15.5, ved hjælp af standard immunologiske farvningsmetoder efterfulgt af optisk clearance og konfokal mikroskopi. Denne teknik muliggør visualisering af blodkar i hele hjertet uden behov for tidskrævende analyse af serielle sektioner.

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Developmental Biology koronarkar Babb PECAM1 embryonale hjerte wholemount immunfluorescens optisk clearing konfokal mikroskopi
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