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Developmental Biology

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JoVE Journal Developmental Biology
Analyse van de coronaire vaten in Gewist Embryonale Hearts
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Analyse van de coronaire vaten in Gewist Embryonale Hearts

Article DOI: 10.3791/54800-v • 08:25 min • December 7th, 2016
December 7th, 2016 •
1, 1, 2, 1
1Developmental Biology of Birth Defects, UCL Institute of Child Health, 2MRC Centre for Regenerative Medicine, SCRM Building, University of Edinburgh

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We presenteren een protocol voor de analyse van coronairvaten geheel muriene embryonale hart tot E15.5, met behulp van standaard immunologische werkwijzen kleuring gevolgd door optische klaring en confocale microscopie. Deze techniek maakt visualisatie van bloedvaten door het gehele hart zonder dat tijdrovende analyse van seriële secties.

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Developmental Biology coronaire vaten BABB PECAM1 embryonale hart wholemount immunofluorescentie optische clearing confocale microscopie
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