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Journal / Developmental Biology / Analys av kranskärlen i clearade embryonala…
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Developmental Biology

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JoVE Journal Developmental Biology
Analys av kranskärlen i clearade embryonala hjärtan
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Analys av kranskärlen i clearade embryonala hjärtan

Article DOI: 10.3791/54800-v • 08:25 min • December 7th, 2016
December 7th, 2016 •
1, 1, 2, 1
1Developmental Biology of Birth Defects, UCL Institute of Child Health, 2MRC Centre for Regenerative Medicine, SCRM Building, University of Edinburgh

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Vi presenterar ett protokoll för analys av kranskärlen i hela embryonala murina hjärtan upp till E15.5, användning av standardimmunologiska färgningsmetoder följt av optisk clearance och konfokalmikroskopi. Denna teknik möjliggör visualisering av blodkärl i hela hjärtat utan behov av tidsödande analys av seriella sektioner.

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Utvecklingsbiologi kranskärlen BABB PECAM1 embryonal hjärta wholemount immunofluorescens optisk clearing konfokalmikroskopi
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