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Neuroscience

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JoVE Journal Neuroscience
Udarbejdelse af Primære Mixed Glia kulturer fra Voksen Mouse Spinal Cord Tissue
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Udarbejdelse af Primære Mixed Glia kulturer fra Voksen Mouse Spinal Cord Tissue

Article DOI: 10.3791/54801-v • 07:13 min • November 19th, 2016
November 19th, 2016 •
1, 1
1Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England

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Udviklingen af ​​neuropatisk smerte omfatter patologiske ændringer i rygmarven gliaceller. En pålidelig glia kultur-system stammer fra voksen rygmarv væv og designet til at studere disse celler in vitro mangler. Derfor viser vi her hvordan man etablerer primære blandede gliale kulturer fra voksne mus rygmarven væv.

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Neuroscience primær blandet glial kultur rygmarv voksen gnaver papain densitet-gradient medium neuropatisk smerte
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