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JoVE Journal Developmental Biology
Visualisera Interrenal steroidogena Tissue och dess Vascular mikromiljö i Zebrafish
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Visualisera Interrenal steroidogena Tissue och dess Vascular mikromiljö i Zebrafish

Article DOI: 10.3791/54820-v • 07:19 min • December 21st, 2016
December 21st, 2016 •
1, 1, 1, 1
1Department of Life Science, Tunghai University

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Den interrenal körtel i zebrafisk är teleostean motsvarigheten till däggdjur binjuren. Detta protokoll beskriver hur du utför tre-β-hydroxisteroiddehydrogenas (Δ 5-4 isomeras, 3β-HSD) enzymatisk aktivitet analys som detekterar differentierade steroidogena celler i utvecklingszebrafisk.

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Utvecklingsbiologi 3-β-hydroxisteroiddehydrogenas zebrafisk steroidogena adrenokortikal interrenal njure blodkärl mikro vibratome sektionering immunohistokemi
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