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December 05, 2016
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Temperature-sensitive (ts) lethal mutants are valuable tools to identify and analyze essential functions. Here we describe methods to generate and classify ts lethal mutants in high throughput.
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Cite this Article
Breker, M., Lieberman, K., Tulin, F., Cross, F. R. High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii. J. Vis. Exp. (118), e54831, doi:10.3791/54831 (2016).
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