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JoVE Journal Genetics
Una sola célula de la expresión génica utilizando multiplex RT-qPCR para caracterizar heterogeneidad de las poblaciones linfoides Raras
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Una sola célula de la expresión génica utilizando multiplex RT-qPCR para caracterizar heterogeneidad de las poblaciones linfoides Raras

Article DOI: 10.3791/54858-v • 10:23 min • January 19th, 2017
January 19th, 2017 •
1, 1, 2, 1, 1
1Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 2Center for Translational Science, Institut Pasteur, INSERM UMS20

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Este protocolo describe la forma de evaluar la expresión de una gran variedad de genes a nivel clonal. Una sola célula de RT-qPCR produce resultados altamente fiables con una fuerte sensibilidad para cientos de muestras y los genes.

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Genética No. 119 de una sola célula la expresión de genes patrón de expresión la heterogeneidad celular linfoides células innatas de microfluidos (CIT)
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