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Biochemistry

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JoVE Journal Biochemistry
Online Grootte-uitsluiting en ionenuitwisselingschromatografie op een SAX Beamline
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Online Grootte-uitsluiting en ionenuitwisselingschromatografie op een SAX Beamline

Article DOI: 10.3791/54861-v • 11:09 min • January 5th, 2017
January 5th, 2017 •
1, 2,3, 4
1Structural Biology Group, European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences, Keele University, 4Groupe de Microscopie Electronique et Méthodes, Institut de Biologie Structurale

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De bepaling van de oplossing structuur van een eiwit door kleine hoek röntgen verstrooiing (SAXS) vereist monodisperse monsters. Hier presenteren we twee mogelijkheden te verzekeren minimale vertragingen tussen monstervoorbereiding en data acquisitie: online size-exclusion chromatography (SEC) en online ionenuitwisselingschromatografie (IEC).

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Biochemie Bio-Small Angle X-ray Scattering (BioSAXS) online size-exclusion chromatografie (SEC) online ionenwisselingschromatografie (IEC) achtergrond aftrekken D5 vaccinia
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