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Journal / Biochemistry / En optimalisert protokoll for Elektro Mobility…
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Biochemistry

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JoVE Journal Biochemistry
En optimalisert protokoll for Elektro Mobility Skift Assay bruker infrarød Fluorescent Dye-merkede oligonukleotider
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Journal (Biochemistry)
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Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
Journal (Biochemistry)
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
 
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En optimalisert protokoll for Elektro Mobility Skift Assay bruker infrarød Fluorescent Dye-merkede oligonukleotider

Article DOI: 10.3791/54863-v • 09:58 min • November 29th, 2016
November 29th, 2016 •
1, 1, 1
1Department of Biological Sciences, University of Illinois at Chicago

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Vi beskriver her en optimalisert protokoll av fluorescerende Elektrofore Shift Mobility Analyser (FEMSA) med rensede SOX-2 proteiner sammen med infrarøde fluorescerende fargestoff-merkede DNA-prober som en case-studie for å takle en viktig biologisk spørsmålet.

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Biokjemi fluoriserende EMSA fluorescerende fargestoff infrarød imaging system, HMG-box transkripsjonsfaktorer postmitotic nevronale identiteter
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