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Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
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Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Article DOI: 10.3791/54870-v • 11:04 min • December 24th, 2016
December 24th, 2016 •
1, 1, 1, 1, 1,2,3
1Department of Chemistry and Biochemistry, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles, 3Jonsson Comprehensive Cancer Center, University of California, Los Angeles

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Summary
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We describe a method for generating localization and affinity purification (LAP)-tagged inducible stable cell lines for investigating protein function, spatiotemporal subcellular localization and protein-protein interaction networks.

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Inducible LAP-tagged Stable Cell Lines Protein Function Spatiotemporal Localization Protein Interaction Networks Molecular Biology Cell Biology High Throughput Analysis Biological Pathways Gene Cloning Transfection HEK293 Cells Hygromycin-containing Media Cell Foci Trypsin
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