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Immunfarvning af biocytin-fyldte og Forarbejdede Sektioner til Neurokemiske Markers
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Immunfarvning af biocytin-fyldte og Forarbejdede Sektioner til Neurokemiske Markers

Article DOI: 10.3791/54880-v • 08:54 min • December 31st, 2016
December 31st, 2016 •
1, 2, 2, 2
1Graduate School of Biomedical Sciences, Rutgers New Jersey Medical School, 2Department of Pharmacology, Physiology and Neuroscience, Rutgers New Jersey Medical School

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Denne protokol udgør en metode til den morfologiske genopretning af neuroner lappet under elektrofysiologiske optagelser med biocytin påfyldning og efterfølgende immunhistokemisk efterbehandle. Vi viser, at tykke biocytin fyldt sektioner, der blev farves og dækglas kan restained med en anden primær antistof dage eller måneder senere.

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Neuroscience morfologi biocytin immunhistokemi neurokemiske markør dobbelt mærkning patch clamp
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