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JoVE Journal Neuroscience
Immunokleuring van biocytine gevuld en Verwerkte secties voor Neurochemische Markers
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Immunokleuring van biocytine gevuld en Verwerkte secties voor Neurochemische Markers

Article DOI: 10.3791/54880-v • 08:54 min • December 31st, 2016
December 31st, 2016 •
1, 2, 2, 2
1Graduate School of Biomedical Sciences, Rutgers New Jersey Medical School, 2Department of Pharmacology, Physiology and Neuroscience, Rutgers New Jersey Medical School

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Dit protocol geeft een werkwijze voor de morfologische herstel van neuronen patched tijdens elektrofysiologische opnames via biocytine vullen en immunohistochemische latere nabewerking. We laten zien dat dikke-biocytine gevuld secties die werden gekleurd en afgedekt kunnen worden restained met een tweede primair antilichaam dagen of maanden later.

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Neuroscience morfologie biocytine immunohistochemie neurochemische marker dubbele etikettering patch clamp
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