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Journal / Developmental Biology / Snabb neuronal differentiering av inducerade…
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Developmental Biology

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JoVE Journal Developmental Biology
Snabb neuronal differentiering av inducerade pluripotenta stamceller för att mäta nätverksaktivitet på Micro-elektrod arrayer
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Snabb neuronal differentiering av inducerade pluripotenta stamceller för att mäta nätverksaktivitet på Micro-elektrod arrayer

Article DOI: 10.3791/54900-v • 09:20 min • January 8th, 2017
January 8th, 2017 •
*1,2, *3, 2,3, 3, 1,2, 1,2, 1,2, 2,3,4, 1,2,3
1Department of Cognitive Neurosciences, Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour, Radboud University, 3Department of Human Genetics, Radboudumc, 4Department of Molecular Developmental Biology, Radboud University
* These authors contributed equally

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Vi ändrar och genomföra ett tidigare publicerat protokoll beskriver snabb, reproducerbar och effektiv differentiering av mänskliga inducerade pluripotenta stamceller (hiPSCs) till excitatoriska kortikala neuroner 12. Specifikt gör att våra modifikation kontroll av neuronal celltäthet och användning på mikroelektrodanordningar för att mäta elektrofysiologiska egenskaper på nätverksnivå.

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Utvecklingsbiologi inducerade pluripotenta stamceller neuronal differentiering mikro-elektrodanordningar lentivirala transduktion astrocyternas isolering neuronala nätverk
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