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Journal / Developmental Biology / Ruimtelijke en temporele analyse van Active ERK…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Ruimtelijke en temporele analyse van Active ERK in de
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Ruimtelijke en temporele analyse van Active ERK in de

Article DOI: 10.3791/54901-v • 08:40 min • November 29th, 2016
November 29th, 2016 •
1, 1,2
1Program in Developmental Biology, Baylor College of Medicine, 2Department of Genetics, UT MD Anderson Cancer Center

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We presenteren een immunofluorescentie-imaging methode voor ruimtelijke en temporele lokalisatie van actieve ERK in de ontleed C. elegans gonade. De hier beschreven protocol kan worden aangepast voor visualisatie van elke signalering of structurele eiwit in het C. elegans gonade, mits een geschikt antilichaam reagens beschikbaar is.

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Developmental Biology RAS-ERK, Kiemlijn, immunofluorescentie signaaleiwitten
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